Journal: American Journal of Translational Research

Article Title: Osteopontin-integrin signaling positively regulates neuroplasticity through enhancing neural autophagy in the peri-infarct area after ischemic stroke

doi:

Figure Lengend Snippet: OPN facilitates neural plasticity and neuronal survival. A. Location of visual field acquisition in the peri-infarct area and corresponding regions in the contralesional hemisphere in mice after stroke. B. VgluT1 immunostaining images in the peri-infarct area and contralateral cortex in stroke+saline and stroke+rOPN groups, n=6 per group. C. Quantification of VGluT1 puncta. D. Representative bands and quantification of SYN in the peri-infarct area after rOPN treatment, n=3 per group. Data are expressed as the mean ± SEM, **P<0.01 vs. stroke+saline group. Original magnification, 40×. Scale bar: 20 μm.

Article Snippet: Then, we used rabbit anti-SYN (1:1000, bs-8845R, Bioss, China), goat anti-OPN (1:1000, ab11503, Abcam, UK), rabbit anti-integrin-β1 (1:1000, bs-0486R, Bioss, China), rabbit anti-beclin1 (1:1000, bs-1353R, Bioss, China), or rabbit anti-β-actin (1:1000, bs-0061R, Bioss, China) as primary antibodies and incubated the membrane overnight at 4°C.

Techniques: Immunostaining

Journal: American Journal of Translational Research

Article Title: Osteopontin-integrin signaling positively regulates neuroplasticity through enhancing neural autophagy in the peri-infarct area after ischemic stroke

doi:

Figure Lengend Snippet: OPN improves axon plasticity through the integrin pathway. A. Immunofluorescence staining images of NeuN and ITGB1 in the peri-infarct area of the stroke+saline, stroke+rOPN, stroke+GRGDSP, and stroke+GRGDSP+rOPN groups, n=6 per group (**P<0.01 vs. stroke+saline group, ##P<0.01 vs. stroke+saline group, &&P<0.01 vs. stroke+rOPN group). B. Western blot of ITGB1 expression in the peri-infarct area after rOPN and GRGDSP injection, n=3 per group (**P<0.01 vs. stroke+saline group, #P<0.05 vs. stroke+saline group, &&P<0.01 vs. sham group). C. Western blot of SYN expression in the peri-infarct area after rOPN, saline, or GRGDSP injection, n=3 per group (**P<0.01 vs. stroke+saline group, ##P<0.01 vs. sham group, &&P<0.01 vs. stroke+rOPN group). Data are expressed as the mean ± SEM. Original magnification, 40×. Scale bar: 20 μm.

Article Snippet: Then, we used rabbit anti-SYN (1:1000, bs-8845R, Bioss, China), goat anti-OPN (1:1000, ab11503, Abcam, UK), rabbit anti-integrin-β1 (1:1000, bs-0486R, Bioss, China), rabbit anti-beclin1 (1:1000, bs-1353R, Bioss, China), or rabbit anti-β-actin (1:1000, bs-0061R, Bioss, China) as primary antibodies and incubated the membrane overnight at 4°C.

Techniques: Immunofluorescence, Staining, Western Blot, Expressing, Injection